Photobleaching with a subnanosecond laser flash

Laser Damage Study With Subnanosecond Pulses

This report describes a ruby laser test facility for conducting laser-Induced damage experiments with single subnanosecond IISBE. The facility includes a laser damape monitoring system and a mode-locked oscillator-amplifier system with pulse selection.

Beyond photobleaching, laser illumination unbinds fluorescent proteins.

Confocal and two-photon fluorescence microscopy techniques using genetically encoded fluorescent probes are widely used in cell biology. Beyond the common problems of photobleaching and phototoxicity, we present evidence that photounbinding also has the potential to compromise such methods, especially in quantitative studies. We show that laser intensities within excitation regimes typical for ...

Subnanosecond mJ eye-safe laser with an intracavity optical parametric oscillator in a shared resonator.

We theoretically verify that the threshold of an intracavity optical parametric oscillator pumped by a passively Q-switched laser is entirely controlled by the bleach of the saturable absorber not by the signal output reflectivity. We use a series of different output couplers to optimize the output performance. With a signal output reflectivity of 15%, we experimentally achieve an efficient sub...

A simple high-voltage high current spark gap with subnanosecond jitter triggered by femtosecond laser filamentation

We describe a simple, sturdy and reliable spark gap operating with air at atmospheric pressure and able to switch currents in excess of 10 kA with sub-nanosecond jitter. The spark gap is remotely triggered by a femtosecond laser filament. Many applications rely on the initiation of a well controlled discharge between two charged electrodes. For high current applications, a gas-filled trigger is...

Fast Gate: Subnanosecond Gated Detectors for Laser Radiography